10220-H08H
Human Complement C1s Protein (His Tag)
Sino Biological
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DESCRIPTION
A DNA sequence encoding the recombinant human complement componentC1s (NP_001725.1) (Met 1-Asp 688) was expressed with a C-terminal polyhistidine tag.
DETAILS
- Tag: C-His
- Purity: > 90 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC
- Source: HEK293 Cells
- Species: Human
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 7,000 pmoles/min/μg, as measured under the described conditions.
- Molecule: C1s
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4.
- Molecular Mass: The recombinant human C1s protease consists of 684 amino acids and has a predicted molecular mass of 76.3 kDa. As a result of glycosylation, rhC1s migrates as an approximately 82.06 kDa, 56.59 kDa and 33.36 kDa band in SDS-PAGE under reducing conditions.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_001725.1
- Predicted N Terminal: Glu 16