Rpt6 is one the six ATPase subunits of the 26S proteasome. The 26S proteasome is assembled from the 20S proteasome and the 19S regulatory particle that contains 19 different proteins, and possesses polyubiquitin binding, deubiquitination, and ATPase activities. The six ATPase subunits of the 19S regulaotry form a ring in a sequence of Rpt1/Rpt2/Rpt6/Rpt3/Rpt4/Rp5. The ATPase ring interacts with the α ring of the 20S proteasome to form the 26S proteasome that degrades polyubiquitinated proteins.