DESCRIPTION

• Category: ZYMO KITS
• Keywords: D4051
• Paragraph 1: The ZR DNA Sequencing Clean-up Kit™ provides a simple method for the rapid removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data. In particular, unincorporated dyes can result in dye peaks (""""dye blobs"""") which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software. DNA is eluted with a small volume of water or loading dye containing formamide. The entire DNA purification procedure typically takes about 2 minutes.
• Bullet Point 1: Complete elimination of """"Dye Blobs"""" for high quality Phred scores and long read lengths
• Bullet Point 2: Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps
• Bullet Point 3: Simple 2 Minute Bind, Wash, Elute Procedure
• Bullet Point 4: Simplest, Most Reliable Method for Dye-Terminator Removal From DNA!
• Manufacturer Sku: D4051