32-1611
Myostatin His Recombinant Protein
Abeomics
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DESCRIPTION
Source : Escherichia Coli. Total 152AA. M.W. 16.7kDa (calculated). N-terminal His-tag & spacer (43AA - highlighted). The AA sequence of the human myostatin part of the fusion protein is corresponding to the UniProtKB/Swiss-Prot entry O14793. Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues & secreted into plasma. Myostatin is a member of the transforming growth factor superfamily of secreted growth & differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development & physiology.
DETAILS
- Amino Acid: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDPSSRSAVR SRRDFGLDCD EHSTESRCCR YPLTVDFEAFGWDWIIAPKR YKANYCSGEC EFVFLQKYPH THLVHQANPR GSAGPCCTPT KMSPINMLYF NGKEQIIYGKIPAMVVDRCG CS.
- Purification: Greater than 95% as determined by SDS-PAGE.
- Product Content: Filtered (0.4µm) & lyophilized from 0.5 mg/ml in 0.05M acetate buffer, pH 4.5.
- Alternative Name: GDF-8||MSTN||Growth/Differentiation Factor 8,MSTN Muscle Hypertrophy.
- Application Note: Add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5 mg/mL & let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.
- Storage Condition: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.