DPP8 Assay Kit

DESCRIPTION

The Fluorogenic DPP8 Assay Kit is designed to measure DPP8 activity using purified DPP8 for screening and profiling applications. The key to the Fluorogenic DPP8 Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP8 reactions. The fluorometric substrate is incubated with a sample containing DPP8 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.

DETAILS

Notes: The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.
Shiptemp: -80°C (dry ice)
Synonyms: Dipeptidylpeptidase 8, MSTP141
Warnings: Avoid freeze/thaw cycles.
Category: Protease/Assay Kit
Background: Dipeptidyl peptidase-8 (DPP8) is a ubiquitously expressed serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP8 shares homology and substrate recognition patterns with DPP4 and like DPP4, is thought to play a role in T-cell activation and immune function.
References: 1. Lee, H.J. et al., J. Biol. Chem. 281 (50), 38653-38662 (2006). 2. Yu, D.M. et al., FEBS J. 273 (11), 2447-2460 (2006). Application Reference(s): Ran, Y., et al. Mol Divers . 2015 May;19(2):333-46. doi: 10.1007/s11030-015-9570-x. Scaffold-based design of xanthine as highly potent inhibitors of DPP-IV for improving glucose homeostasis in DIO mice (2015)
Description: The Fluorogenic DPP8 Assay Kit is designed to measure DPP8 activity using purified DPP8 for screening and profiling applications. The key to the Fluorogenic DPP8 Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP8 reactions. The fluorometric substrate is incubated with a sample containing DPP8 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.
Methodology: Fluorogenic
Supplied As: Supplied in a convenient 96-well format, with purified DPP8 enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions.
Unspsc Code: 41105331
Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Product Type: Assay Kit
Biosafety Level: Not applicable (BSL-1)
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Storage Stability: At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Scientific Category: Protease
Instructions for Use: See assay kit datasheet for detailed protocol.