DESCRIPTION

• Aa: full length
• Mw: 131 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: BC112393
• Species: Human
• Uniprot: Q9HCR9
• Shiptemp: -80°C (dry ice)
• Synonyms: PDE11, phosphodiesterase 11A
• Warnings: Avoid freeze/thaw cycles.
• Category: Phosphodiesterase/Enzymes
• References: 1. Loughney K, et al., Int. J. Impot. Res. 2005, 17(4):320-5.2. Weeks JL, et al., Int. J. Impot. Res. 2005, 17(1):5-9. Application Reference(s): 1. Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014) 2. A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013) 3. The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)
• Description: Human PDE11A (GenBank Accession No.BC112393), full length with N-terminal GST-tag, MW= 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
• Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM glutathione, and 3 mM DTT
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3' , 5' -cAMP to 5' -AMP per minute at 37°C.
• Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2 , 0.1 mg/ml BSA,_x000D_ 200 µM cAMP, 2.5 kU 5'-nucleotidase, and PDE11A at 37°C for 20 min._x000D_ Quantified by 5'-nucleotidase cleaving the 5'-_x000D_ AMP product and releasing the phosphate group_x000D_ which is detected by Malachite Green Reagent.
• Specific Activity: ≥ 1560 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Phosphodiesterase