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Wayne State University Karmanos Cancer Institute Microscopy, Imaging and Cytometry Resources Core

Website:
karmanos.org/MICR
Headquarters:
Detroit, Michigan, US
Company Type:
Academic Core Facility

The mission of the Microscopy, Imaging & Cytometry Resources (MICR) core is to enhance the peer reviewed funded research activities of WSU investigators whose research requires microscopy, imaging resources, flow cytometry, histology and related techniques.

Core Services Available

Confocal Microscopy, including both point scanning (Zeiss LSM 510, Zeiss LSM 410, Zeiss LSM 780, Leica TCS SP5) and spinning disk (Perkin Elmer UltraView) technologies.
Multiphoton Microscopy, including Second Harmonic Generation imaging
Fluorescent Microscopy
Conventional, widefield epifluorescence (Zeiss ApoTome)
Optical sectioning using Structured Illumination (Zeiss ApoTome)
In vivo small animal imaging
In vivo small animal Positron Emission Tomography (PET) imaging
In vivo small animal Single Photon Emission Computed Tomography (SPECT/CT) imaging
In vivo small animal X-Ray imaging In vivo small animal white light, fluorescence and bioluminescence imaging (Carestream In Vivo Xtreme, Carestream MS FX PRO)
Long-term, live cell imaging. All confocal microscopes are outfitted with live cell imaging chambers to control CO2 flow, temperature and humidity
Advanced Image Analysis, including 3D and 4D image reconstruction and quantitative measurements
Cell analysis for data acquired using the benchtop cytometers and the FACSDiVa. These services range from simple one-color analysis of the cell cycle to multi-color, multi-laser analysis requiring expert knowledge of machine setup and color compensation
Cell sorting (operator driven), using the FACSDiVa. The DiVa sorts up to 20,000 cells/second in a single or two-way sort or can deposit cells in wells of a variety of microwell plates using its automated cell deposition unit
Cytometry Data analysis, using any of three offline workstations in the Hudson Webber Cancer Research Center (HWCRC) or one in the Prentis Cancer Research Building that provides access to licensed Cellquest, DiVa and FlowJo software for basic analysis and programs such as Modfit for cell cycle analysis. The researcher can undertake analysis after suitable training, or Core personnel will provide the service
Expert consultation, including advide on the choice of application and experimental design — contact us here
Training is provided for every new user as well as refresher training for more experienced users, and workshops are provided, as announced
On site support for use of equipment

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Wayne State University Karmanos Cancer Institute Microscopy, Imaging and Cytometry Resources Core
4100 John R Street Detroit, MI, 48201 United States
Wayne State University Karmanos Cancer Institute Microscopy, Imaging and Cytometry Resources Core
4100 John R Detroit, Michigan, 48201 United States

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