Isolation of cells from tissue sections. We developed a well validated protocol for analysing gene expression profiles in subsets of cells in complex tissues from animal and human origin.
Using laser dissection microscopy, we isolate predestined cells from tissue sections and subject their RNA to quantitative RT-PCR analysis. It provides a unique way to unmask the molecular control of cell behaviour in different compartments of healthy and diseased tissues. Also, the effects of therapeutic intervention can be analysed at the molecular level in the cells you are interested in.