The DNA Methylation Analysis Facility is funded by the Center for Cancer Epigenetics and provides services related to the analysis of DNA methylation for researchers across all MD Anderson campuses and in the Texas Medical Center. Services include: consultation with investigators to chose the best method of analysis; sample preparation, assay design and processing of the most used methods to study DNA methylation, both gene-specific and genome-wide (PyroMeth, RRBS and Methyl-Seq); developing and maintaining a library of commonly used assays to measure global DNA methylation (LINE, Alu) and common gene-specific tests (p16, e-cadherin, MLH1, p15 and others). The core is located at the Clinical Research Building of the MD Anderson Cancer Center in Houston. We are fully equipped for molecular biology research and have a Qiagen Pyrosequencing machine.