The antibody core facility purifies by affinity chromatography antibodies specific for cell differentiation markers, signalling and tagging molecules of various species. We conjugate these antibodies to various fluorochromes (fluorescein, allophycocyanin, phycoerythrin, and up to 20 different Alexa Fluor dyes), to biotin, and to microbeads for cell surface multicolour immunofluorescence, immunohistochemistry, functional assays and biochemical analyses.
Following purification and conjugation of an antibody, the optimal titer for various applications is determined.
Aside from antibodies, the facility also produces other reagents needed for various molecular biology techniques. Please contact Philippe Poussier for more information.
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University of Toronto FoM Sunnybrook Research Institute Antibody Core
2075 Bayview Avenue, A3 38
Toronto, ON, M4N 3M5
Canada
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