University of Florida CTSI SECIM Mass Spectrometry Services
Website:
secim.ufl.edu |
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Headquarters:
Gainesville, FL, US |
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Company Type:
Academic Core Facility |
Core 1 provides high-throughput global and targeted metabolomic and lipidomic analysis. For further details on prices and to request services, CLICK HERE.
Global metabolomics and/or lipidomics is performed using state-of-the-art high resolution mass spectrometry (Thermo Q-Exactive) coupled with ultra-high performance liquid chromatography (UHPLC) on serum, plasma, urine, tissue, cells, stool and other samples.
Targeted metabolomics offerings include:
Acylcarnitines
Amino acids
Organic acids
Malonyl CoA and Acetyl CoA
Pyridine Nucleotides (NMN, NAD, NADH, NADP, NADPH)
Tryptophan Metabolites
1-Carbon metabolites
These are performed for a variety of matrices including cellular extracts, plasma or tissue using mass spectrometry. New assays will be developed based on:
Demand and/or interest in other metabolite subsets and
Follow-up method development to validate putative metabolites identified by prior global metabolomics pursuits.
The targeted metabolomics employs MS with selected reaction monitoring (SRM) using either a direct infusion approach or after chromatographic separation (UHPLC or GC).
Biomarker identification is performed with accurate mass and fine mass resolution, tandem MS (MS/MS) provided by Core 3 and existing metabolomic databases (Metlin, ChemSpider, pubChem). Through constant collaboration, SECIM continues to grow the library of known metabolites.
Special emphasis in Core 1 is placed on developing SOPs for proper collection/storage of specimens and sample processing prior to MS and NMR. However, interaction with Core 3 provides a mechanism to improve the overall global metabolomics platform as new technologies are developed. An example of this is Isotopic Ratio Outlier Analysis (IROA), a collaborating technology offered through SECIM services.
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University of Florida CTSI SECIM Mass Spectrometry Services 2004 Mowry Road Gainesville, FL, 32603 United States | ||
University of Florida CTSI SECIM Mass Spectrometry Services 2004 Mowry Road Gainesville, FL, 32603 United States |
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