Well-differentiated human airway epithelial cell cultures are instrumental for studying basic and applied aspects of cell biology and physiology relevant to cystic fibrosis (CF) and its treatment. The The Marsico Lung Institute (MLI) Tissue Procurement and Cell Culture Core (TPC) was established to provide standardized cell cultures for CF research. The TPC serves as a central source of normal, CF and disease control cells, tissues and fluids for a wide array of uses. The TPC has prepared cells from approximately 7,300 human tissue specimens over the past 30 years, providing an average of 13 × 109 viable cells per year over the past four years.