Stellate DNA provides a novel class of high quality, sequence verified, endotoxin-free DNA donor (Genome Catalyst or Gatalyst™) as templates for CRISPR-mediated gene integration (Knock-In or KI). The Stellate Gatalyst™ donor is in a proprietary construction of a circular form of single strand DNA circular single-stranded DNA (cssDNA), which stimulates integration by homologous repair (HR) at the correct integration sites while minimizing the non-homologous integration of the donor into other genome loci or error prone repair via non-homologous end-joining.