The Flow Cytometry Core Lab provides support to the NJMS research community as well as to surrounding universities and private companies for applications involving flow cytometric analysis, cell sorting and imaging flow cytometry. Resources available -
Flow cytometric analysis:
•Three BD Biosciences FACSCaliburs each having a dual laser capacity with capabilities to do up to four color analysis and an HTS sampler for multiwell sampling.
•LSR II air-cooled four-laser benchtop flow cytometer with the ability to acquire 12 colors.
Cell sorting:
• BD FACSAria II SORP: The FACSAria II is equipped with four lasers allowing for 12 marker based sorting for clonal expansion, multi-well plating, sorting onto slides and simultaneous 2, 3, or 4 population sorting.
•BD FACSAria II (BSL-3): allows for the analysis and sorting of live TB and other agents that require BSL-3 handling along with the ability to analyze the interaction between mammalian cells and those pathogens. The FACSAria II is equipped with two lasers allowing for simultaneous acquisition of up to 10 parameters.
Imaging Flow Cytometry:
•Amnis ImageStream imaging flow cytometer that is equipped with 488 nm and 658 nm lasers and an extended depth of field module. This instrument, which operates in a Windows-based environment, introduces a new technology platform that captures high-resolution digital images of cells in flow at rates of approximately 100 cells/per second.
Software resources :
•Additional software available for use in multiparameter studies includes BD Bioscience CellQuest for the Caliburs, Modfit LT software for DNA and cell proliferation analysis, HTS software for the HTS sampler, and BDDiva software for the LSRII and the Cell sorter.
•The Core Laboratory also adminsters a university site license for FlowJo analysis software.