The Werner Straus Live Cell Imaging Laboratory is a core facility in the Department of Cell Biology and Anatomy that offers access to state-of-the-art, high-speed multidimensional image analysis specifically designed for living cells. The laboratory provides expertise in light imaging techniques that include 4D data acquisition, FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss In Photobleaching) and FRET (Fluorescence Resonance Energy Transfer). These experimental approaches are widely used to study molecular dynamics in live cells, highlighted by the use of green fluorescent protein and its variants to visualize protein trafficking. The facility houses a Nikon Imaging System and a Zeiss LSM 510 Confocal System, which are available on a fee-for-use basis (see below). A separate off-line PC workstation with Metamorph and AutoQuant Deconvolution software and the Zeiss LSM software is available for post-acquisition image analysis and data processing. Specific arrangements must be made with the Lab Directors before projects are initiated. In-house training will be given to all new users and consultation regarding imaging aspects of your research project will be provided. Investigators will supply their own samples and materials.