We are pleased to announce the creation of the Molecular Quantitation Laboratory (MQL). The MQL, situated on the second floor of the basic science building, is equipped with a new Applied Biosystems (ViiA7) quantitative real-time polymerase chain reaction (qRT-PCR) machine. This instrument will be available for accurate and sensitive high-throughput measurements of gene expression. This instrument will also be equipped with Protein Thermal Shift technology for analysis of protein stability and binding interactions. This new technology can be used for high-throughput screening of proteins to identify ligands, mutations/modifications, or buffer conditions to increase melting temperature and the relative stability of proteins. This application can also screen antibody–ligand binding as part of antibody development, or be used to assess protein preps for contaminants or aggregated protein.
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Rosalind Franklin University of Medicine and Science Molecular Quantification Laboratory
3333 Green Bay Road
North Chicago, IL, 60064
United States
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Rosalind Franklin University of Medicine and Science Molecular Quantification Laboratory
North Chicago, IL, 60064
United States
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