Immunofluorescence microscopy is used to image a specific molecule in cells or tissue sections by using a specific antibody conjugated with a fluorescent dye. Currently the facility has two upright microscopes and one inverted microscope (Nikon 80i) with metamorph image analysis software. Available filters for imaging include green, red, blue and cyan fluorescent dyes. The upright microscope is also equipped to perform phase contrast and differential interference contrast (DIC) image analysis.
General applications include visualization of Alexa Fluor 350, 405, 488, 594, Texas red, FITC, GFP, DAPI, Z-section using automated motors and quantification of colocalised proteins.
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Rosalind Franklin University of Medicine and Science H. M. Bligh Fluorescent Microscopy
3333 Green Bay Rd
North hicago, IL, 60064
United States
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Rosalind Franklin University of Medicine and Science H. M. Bligh Fluorescent Microscopy
3333 Green Bay Road
North Chicago, IL, 60064
United States
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