Advancing your research from cloning to characterization. Our mission is to provide value-added services to enable our clients to accelerate their product development programs and build long-lasting collaborative relationships.

POKProtein was built on the premise that excellent science depends on excellent materials. With decades of experience in the pharmaceutical industry we understand the value of outsourcing key tasks, but also the importance of quality and communication. POKProtein is a Contract Research Organization, specialized in recombinant protein expression, purification, and characterization.

We are proud to offer below services to all biotech and pharmaceutical companies globally:

Protein Production and Characterization
Cloning

Protein Expression in E.coli, Baculovirus and Mammalian cell lines

Protein Purification
Standard protein purification methods
Protein denaturing and refolding techniques
Affinity purification: IMAC, GST, FLAG, Myc, HA, heparin, phospho-cellulose, antibody matrices and custom matrices
Classic chromatography: ion exchange, hydrophobic interaction, size exclusion, dye-ligand regular and reverse-phase HPLC

Protein characterization:
SDS-PAGE
Western blotting
Thermal shift assays
HPLC
Purity
Aggregation analysis
Concentration measurement
Titer analysis
Binding analysis
Analytical SEC
SEC-MALS
Intact Mass
Identity and Sequence Confirmation
Post Translational Modification (PTM) analysis
N-Glycan analysis

Binding assessment using SPR/Biacore
Binding kinetics and affinity
Off target binding
Species cross reactivity
Target family members
Cross reactivity
Protein domain screening
Ligand/ Receptor Blocking
Cross blocking
Sandwich assays
Uni or Bidirectional epitope binning
FcγR and FcRn binding
Thermodynamic analysis
Active concentration