Pelago’s patented CETSA® technology measures a compound’s on- and off-target engagement in a physiologically relevant cellular environment. CETSA® is a label free technology that measures the change in a protein’s thermal stability induced by compound binding. The method is suitable for material from any source, cells, lysates, animals and human biopsy material. CETSA® can be applied against a specific protein target or in an unbiased proteome-wide assay to profile a compound’s activity against up to 8000 proteins.