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Iowa State University qPCR Consultation Service

Website:
biotech.iastate.edu/biotechnology-service-facilities/qpcr-consultation-service
Headquarters:
Ames, IA, US
Company Type:
Academic Core Facility

The qPCR Consultation Service provides consultation for comprehensive qPcR assay design assistance. All steps are discussed and/or printed out as easy-to-follow processes for immediate in-lab use.

Services Offered:

Basic information/qPCR theory and math
Primer-probe design assistance
Identification of appropriate reagents, master mixes and machine platforms for One- and Two-Step qPCR
MIQE-aware methodology and guideline concerns, DNAse treatment and reverse transcription reaction formulation suggestions and guidance
Nucleic acid quality assessment and quantity measurement suggestions and guidance
Processing of global assay parameters using ISURF software #03407 (PREXCEL-Q)
Consultation regarding detection and avoidance of RT and PCR inhibition for all sample types and isolation methods
File system creation and initial qPCR test plate set-up printouts and folow-up consultation
Processing of test plate results into final set-up parameters and procedural printouts for final sample qPCR
Excel spreadsheets custom-created for EAMP-corrected data analysis and graphing
All plant and animal species are considered. Fees for services are $90 per hour. Please email or call one week in advance to set up a consultation.

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Iowa State University qPCR Consultation Service
1520 ISU C Drive Ames, IA, 50011 United States
Iowa State University qPCR Consultation Service
1520 ISU C Drive Ames, IA, 50011 United States

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