The Protein Facility provides equipment and expertise for the analysis, characterization and synthesis of proteins and peptides and is open to both internal and external investigators. The Protein Facility is supported by the Office of Biotechnology, the Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology and the Plant Science Institute’s Center for Plant Genomics. The following instrumentation is available in the facility:

Amersham Pharmacia DALT 2D electrophoresis system
Amersham Pharmacia Image Scanner with transparency module
Applied Biosystems Model 494 Procise protein/peptide sequencer
Applied Biosystems DE-Pro MALDI mass analyzer
Beckman System Gold high-performance liquid chromatographs
Model 125A Analytical Solvent Module with Model 166 programmable UV detector
Model 110A Semi-prep/prep Solvent Module with Model 166 programmable UV detector
Model 125S Microanalytical Solvent Module with Model 166 programmable UV detector and SC100 fraction collector
Beckman Coulter P/ACE MDQ Capillary Electrophoresis System
Biorad minigel and blotting apparatus
C.B.S. Scientific spot-picking workstation with UV lightbox
GE Healthcare Ettan IPGPhor 3 IEF unit
GE Healthcare Typhoon FLA 9500 Scanner
Genomics Solutions ProGest
NanoDrop ND1000 Spectrophotometer
Savant SpeedVac Plus
Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer coupled with an Agilent 1260 Infinity Capillary pump
Virtis Model 3.5L DBTZL Benchtop FreezeDryer (lyophilizer)
Software for the analysis of 1D and 2D gels

The following services are offered.

High Performance Liquid Chromatography (HPLC)
MALDI-TOF Mass Spectrometry
Peptide Synthesis
Protein/Peptide Sequencing
Tandem Mass Spectrometry (LC-MS/MS)
In-gel Digestion/Peptide Mass Fingerprinting
SDS-PAGE/Electroblotting
2-D Gel Electrophoresis
Isoelectric Focusing (IEF)
2-D Gel Analysis
Image Scanning and Analysis