Reverse Phase protein Arrays represent an innovative technology that allows studying protein expression levels and the activation status of cell signalling pathways. It combines high throughput analysis with minimal sample consumption. Using a dedicated arrayer, only 1 ng of proteins extracted from tissues or cell lines are printed onto nitrocellulose covered microscope slides (arrays). Proteins of interest are then quantified using highly specific primary antibodies. Up to one thousand samples can be analysed simultaneously on the same array. We systematically print serial dilutions and replicates for all samples, making the technology highly robust and quantitative.

Our platform has validated almost 600 primary antibodies for RPPA labelling so far, among which almost 150 antibodies against phosphorylated proteins. This panel of antibodies covers the majority of cell signalling pathways. For each project, the project leader selects the antibodies of interest for his/her project among the validated antibodies. New antibodies are continuously being tested in the context of ongoing scientific projects.