The generation and analysis of genetically modified mice, including transgenic and knockout animals, is an essential technology for cancer research. The Transgenic Mouse Shared Resource makes these powerful technologies available to all Cancer Center members.
Services include:
Production of transgenic mice by pronuclear DNA microinjection and embryo transfer.
Production of chimeric mice by blastocyst injection of genetically modified ES cells and embryo transfer, which is part of the procedure of producing knockout or knockin mice.
CRISPR/Cas9 gene editing in mouse embryos
Gene targeting, which involves the electroporation of a targeting vector into pluripotent mouse embryonic stem (ES) cells and the selection of gene-targeted clones.
Education of investigators and advice on vector and experimental design for experiments using transgenic and knockout mice.