Shared by the Medical College of Wisconsin Cancer Center and Children’s Research Institute, the Flow Cytometry Core is an advanced technology facility that assists investigators with evaluating cell populations based on surface and intracellular antigen expression, apoptosis, bead-based cytokine detection, and cell cycle analysis. Although the facility is primarily used by researchers at MCW and CRI, it’s also available to collaborators at other institutions.

Services include:

Access to multiple cytometer and cell sorting instruments
Training on instrumentation
Cell-sorting services
Consultation to investigators regarding experiment design and protocol development based on instrument capabilities. The facility can advise antibody and fluorochrome selection based on targets of interest, appropriate compensation controls when needed, and provide software support for data analysis.
After training by the facility’s staff, users may run their own experiments on the Calibur and LSR II instruments. Experiments requiring cell sorting are scheduled and executed by the facility staff on the Aria Special Order Systems.