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Carolinas Medical Center Cannon Research Center Mass Spectrometry and Proteomics Core

Website:
carolinashealthcare.org/research-clinical-trials/mass-spectrometry-proteomics-facility
Headquarters:
Charlotte, NC, US
Company Type:
Academic Core Facility

The Mass Spectrometry and Proteomics Core Facility is located in the Cannon Research Center at Carolinas Medical Center. It is a fee-for-service facility dedicated to providing proteomics support to the Carolinas HealthCare System, University of North Carolina at Charlotte, and other non-profit research organizations in the greater Charlotte region. The goal of the core is to facilitate proteomics analysis of clinical and biological samples by offering state-of-the-art instrumentation and proteomics expertise in preparing, analyzing, and bioinformatic interpretation of experimental data.
The current mass spectrometry instrumentation includes an LTQ-XL and a TSQ Quantum Ultra, both with acquity UPLC systems, and an LTQ-XL Orbitrap-ETD with nano acquity UPLC. Additionally, we have six data analysis workstations.. Software platforms are used for proteomic data analysis include the Bioworks SEQUEST and Proteome Discoverer MASCOT search engines, Sieve and Scaffold Q+S for data compilation and quantification, PEAKS for de-novo sequencing, and the in-house script Intersect for use in comparison of more than two groups of samples. Pathway analysis is carried out using Metacore software.
The Mass Spectrometry and Proteomics Core Facility uses high-throughput nano flow liquid-chromatography tandem mass spectrometry to identify proteins from one dimensional or two dimensional polyacrylamide gel slices. Sample preparation includes electrophoresis of the sample, gel staining and excision of regions of interest. The proteins are then digested within the gel slices using trypsin. Resulting peptides are extracted from the gel matrix and analyzed using reversed phase nano-litter flow liquid chromatography followed by tandem mass spectrometry. Another option for analysis is a technique commonly referred to as MudPIT (Multi-dimensional Protein Identification Technology), which incorporates strong cation exchange as an additional dimension of peptide separation. Spectra are analyzed using the software tools Bioworks, Scaffold, PEAKS for identification of peptides and compilation of experimental data.

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Carolinas Medical Center Cannon Research Center Mass Spectrometry and Proteomics Core
1000 Blythe Boulevard Charlotte, NC, 28203 United States
Carolinas Medical Center Cannon Research Center Mass Spectrometry and Proteomics Core
1000 Blythe Boulevard Charlotte, NC, 28203 United States

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