Bioexpert is an independent provider of research services in the life sciences. The company takes in charge the practical steps of execution of the projects. This company works in the Paris region. Life sciences specialist: in vitro and in vivo experiments.
Non-exhaustive list of services:
Management and training of teams for the realisation of projects
Animal experimentation (mouse, rat):
Chemo-induced cancer model,
Xenogenic cancer model,
Inoculations: Intraperitoneal, Intramuscular, Subcutaneous, Intravenous, Intra ocular injections; -Gavage
Samples : Blood, gametes, bone marrow, aorta, aqueous humour, retina, pigment epithelium, choroid, iris and ciliary body, etc.
Genotyping and management of genetically modified mice and breeding management,
Fertility studies,
Behavioural testing
Cell biology :
Cell culture: cell lines, primary cultures: endothelial stem cell, mesenchymal stem cell
Cytotoxicity tests
In vitro angiogenesis tests – on endothelial cells: clonogenicity test, secondary/tertiary colony formation test, functional tests: Migration, Tube formation, Wound healing
Marker studies
Immunofluorescence (Flow Cytometry, microscopy) Immunohistochemistry and Biochemistry (Immunoprecipitation, Western Blot), ELISA,
Production of monoclonal antibodies against surface molecules:
Production of monoclonal antibodies against surface molecules: Production by transfection of a high producing line of the antigen of interest, purification by tag, immunisation of mice/rats, fusion, selection, obtaining monoclonal hybridomas and production/purification of the desired antibodies
Coupling of antibodies with fluorochromes
Study of platelet functions: Aggregation, degranulation – secretion, activation, adhesion and spreading, bleeding time, hemodynamic thrombosis formation, FeCl3 model thrombosis,