Axxam is leading provider of integrated services in early drug discovery across the entire Life Science industries: Pharmaceutical, Crop protection, Animal health, Cosmetics and Nutrition.

Our work to identify bioactive molecules with the desired pharmacological activity, such as the modulation of a target or a cellular pathway, is fundamental to pave the way toward the development of new drug compounds.

Service overview:

- Assay development (biochemical or cell-based, including molecular interactions & PROTAC) and phenotypic assays for compound testing activities and HTS.

- Ready for use assays (functional & phenotypic) for compound testing activities and HTS.

- High Throughput Screening (HTS) campaigns using client’s own compound collections or our compound collection of around 425.000 diverse small molecules (divided into 6 subsets which can be screened separately according to the project’s needs).

- High Content Screening (HCS) campaigns (on up to 20.000 compounds) and Phenotypic analysis: autophagy, neurite outgrowth, cell cicle, citotoxicity, mitochondrial analysis, marker localization, DNA damage, translocation, aggregation assays.

- Molecular biology: gene editing, gene expression analysis, TaqMan-based screening, splice modulators and promoter activation assays.

- iPSC-derived cells (neurons, cardiomyocytes, microglia cells, myotubes, hepatocytes, macrophages): calcium oscillation, aggregation assays, imaging, electrophysiology, cytokine release, citotoxicity, disease models.

- Electrophysiology: manual patch clamp, automated patch clamp suitable for HTS, organellar patch clamp (lysosomes, mitochondria, nuclei), automated lysosomal patch clamp, optogenetics.

- Hit validation & hit-to-lead: potency determination, mechanism of action studies, cardiac safety, specificity, selectivity, ortholog and orthogonal assays, among others.

- Biophysical tools: affinity measurement, Kd determination, spectral shift, molecular interactions, microscale thermophoresis.

- Analytical chemistry: LC-MS analysis, in vitro ADME (plasma permeability assay; plasma protein binding; microsomal stability assay); physico-chemical properties (solubility; lipophilicity; chemical stability).

- Medicinal Chemistry and computational chemistry (analog synthesis; SAR analysis; IP positioning analysis; database maintenance; etc.).

- Compound management: external libraries reformatting & storage.

- AXXSense platform (https://axxsense.com/): specialized in the generation and development of novel, natural and synthetic flavour and fragrance compounds for the food and beverage, pet food, animal health, cosmetic and perfume industry. Taste and olfactory receptors, natural product library, aging platform.