Advancing Gene and Cell Product Development Through iPSC and Genome Editing Technologies

Applied StemCell strives to advance gene-editing and stem cell innovation for biomedical research and the biotechnology industry to assist in the development of breakthrough therapeutic approaches to cure intractable diseases.

1. GMP iPSCs / MSCs Cell Manufacturing
• GMP iPSC Services & Products - Reprogramming, Genome Editing, & Differentiation/cGMP Grade iPSC Control Line Available
• GMP MSC Purification, Expansion and Banking
• iPSC-TARGATT™-CAR-NK Differentiation - Knock in your CAR gene into our control iPSCs and differentiate into NK or T Cells

1. iPSCs, Stem Cell Research Services & Products:
• iPSC Generation & Characterization
• iPSC Genome Editing (CRISPR & TARGATT™) - Off-the-shelf iPSC-genome edited products are available for purchase
• iPSC Differentiation, including T Cells, NK Cells, HPCs, Monocytes, RPEs, Photoreceptors, Endothelial Cells, Oligodendrocytes, NPC, Neurons, Astrocytes, DOPA, Microglia, Cardiomyocytes, and Skeletal Muscle) - Control iPSC-derived items are available
• Disease Modeling and Drug Toxicity Screening (BBB testing), Cytotoxicity Testing
• Available “Master” control iPSCs that have proven genome-editing/differentiation capabilities. Currently, it is being used as a benchmark material for the development of measurements and standards for rapidly evolving genetically engineered cells used in cell therapy research.

2. Animal Model Development Service:
• PBMC and CD34 Mouse Models (Pre-screened, pre-injected)
• Mouse Models and Rat Models
• Site-specific Knock-in Mouse Service (< 22kb) – TARGATT™
• Mouse Repository of 2000+, includes 50+ humanized mouse models and Cre.
• New COVID-19 Humanized Mouse Models
• In Vivo Antibody/Drug Candidate Testing

3. Antibody Discovery & Bioproduction:
• Antibody Discovery: TARGATT™ Cell-Based Antibody Library (HEK293/CHO), TARGATT™ Master Cell Line HEK293 & Rabbit Monoclonal Antibody Development
• Bioproduction: Bioproduction (CHO) & Transgenic Animals-Rabbit

4. Tissue Samples & Primary Cells
• Human Primary Cells: PBMCs, CB
• Human Hematologic Tissue Products: WB procurement
• Human Diseased Products
• Non-Human Primate Cells & Hematologic Tissue Products
• Custom FFPE Service (block/slide/scroll) – Cell Pellet and Tissue
• FFPE Products – Human & Animal Cell Pellets, Human & Animal Tissues
• Cytokines & Growth Factors
• Custom Primary Cell Isolation Service
• Tissue Collection

5. CRO/Research Cell and Gene Therapy Bioservices
• Cell-Based Assay: CAR-NK and CAR-T Assays, AAV, Potency assays, IND-Enabling Studies, Cellular Viability & Proliferation, Migration Studies & ELISA/ELISpot
• In Vivo/Mouse Assay Services: Efficacy Service with HU Models, Dose-Ranging & Biodistribution
• TARGATT™ Knock-in Cell Line
• Lentivirus Stable Cell Line Generation
• Immortalization Services
• Cell Line Irradiation Service
• Virus Packaging
• CMC Support
• HLA typing

Take advantage of our proprietary TARGATT™ gene knock-in technology and HEK293/CHO-K1 systems for high-efficiency knock-in of large transgenes at safe harbor loci (600x times*) to generate your own library for antibody discovery/screening.