Altis Biosystems has pioneered the creation of Repligut®Systems, a collection of in vitro models derived from stem cells and designed specifically for pre-clinical drug discovery. RepliGut® models are designed to replicate the complex structure of the human small intestine and colonic epithelium using a standard user friendly format.

RepliGut® Systems are a series of human primary intestinal epithelial cell model systems that recreate the gastrointestinal cell barrier in standard culture well formats. Using the same starting cellular material as organoids, these models deliver more reproducible assay results with consistent culture timelines across a range of pharmacological applications including inflammation, toxicity, bioavailability, and barrier function.

RepliGut Systems:
1) Replicate human biology using human donor stem cells to recreate different regions of the intestinal epithelium in vitro.
2) Deliver physiologically relevant human data for greater confidence in decision making earlier in the drug discovery process and reducing the need for animal studies.
3) Unlike organoids, RepliGut® model’s unique membrane supported configuration grants access to basal and apical compartments in all experimental phases.

Kits:
RepliGut® Kits are available in both 12-well and 96-well Transwell plate formats to accommodate scientist’s needs. Kits are sold complete with the cells, plates, and media needed to culture this unique human intestinal epithelial cell model in your own facility. Technical support is available.

Services:
All service projects are led by scientists with extensive experience in advanced cell culture systems. Assays are customizable based on your needs and can be run using standard or customized cell culture conditions.

Biobank:
Our biobank consists of multiple intestinal regions from multiple donors, allowing researchers to study colon or small intestine-specific biology in demographically diverse backgrounds (e.g. age, sex, blood type). Our cryopreserved biobank enables the ability to work with the same donor as your project progresses