The Albert Einstein College of Medicine Analytical Imaging Facility (AIF) provides a comprehensive light and electron microscope imaging facility dedicated to offering current methods in modern imaging to biomedical scientists with all levels of expertise. The AIF staff has been cross-trained to seamlessly support visual analysis using techniques ranging from fluorescence light microscope imaging in 3D to high-resolution electron microscopy. This integrated approach facilitates the efficient and appropriate complementary application of these methods for investigators.