Need pure mRNA but want to DIY? Messenger Bio's Embark™️ mRNA Purification Kit is designed for rapid and reliable purification of mRNA after in vitro transcription. The Embark™️ kit enables removal of all major reaction contaminants, including dsRNA contaminants which almost all other kits fail to remove. Each kit comes with: - 8 M Lithium Chloride for isolating mRNA after transcription - 25, 50 or 100 pre-prepared purification columns - mRNA Purification Buffer - 3 M Sodium Acetate (pH 5.2–5.5) for isolating mRNA after purification (you will need to supply your own isopropanol) - 1 mM Citrate Buffer (pH 6.5) for resuspension of mRNA after isolation “I have been using this kit to remove ds-RNA contaminants as I inject the mRNA into mice. I had seen before that using traditional kits led to non-specific immune response as observed by substantial increase in cytokines like IL-6. Using this kit, the issue seems to be resolved as ds-RNA contaminants are removed.” - Chaitanya Naimesh Parikh, UMass Chan Medical School