HIV TAT specific factor 1 (HTATSF1) Human siRNA Oligo Duplex (Locus ID 27336)

DESCRIPTION:

HTATSF1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

DETAILS

Note: Single siRNA duplex (10nmol) can be ordered.
Purity: HPLC purified
Refseq: NM_001163280, NM_014500
Status: 3 Weeks*
Summary: The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Sep 2009]
Category: RNAi
Synonyms: dJ196E23.2; TAT-SF1; TATSF1
Sequences: Available with shipment
Stability: One year from date of shipment when stored at -20°C.
Components: HTATSF1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 27336)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml Need an individual siRNA?
Uniprot Id: O43719
Availability: 3 Weeks
Quality Control: Tested by ESI-MS
Performance Guaranteed: OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
Number Of Transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).