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UC3-H5141-100ug UC3-H5141-1mg

Human UCH-L3 / UCHL3 Protein, His Tag

ACROBiosystems

DESCRIPTION

Human UCH-L3, His Tag (UC3-H5141) is expressed from E. coli cells. It contains AA Glu 2 - Ala 230 (Accession # P15374-1).

DETAILS

  • Tag: N-6×His
  • Host: E. coli
  • Size: 1mg
  • Buffer: 50 mM Tris, 150 mM NaCl, pH7.5
  • Format: Powder
  • Purity: 95%
  • Storage: -20℃
  • Category: Enzymes
  • Molecule: UCH-L3
  • Accession: AAH18125.1
  • Conjugate: Unconjugated
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCH-L3), a member of peptidase C12 family, is also known as ubiquitin thioesterase L3. Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. UCH-L3 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is also required for stress-response retinal, skeletal muscle and germ cell maintenance. Furthermore, UCH-L3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.
  • Exp Region: Glu 2 - Ala 230
  • Swiss-prot: P15374-1
  • Characteristics: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE).
  • Endotoxin Level: 1.0 EU per μg
  • Molecular Weight: 27.0 kDa
  • Shipping Condition: RT
  • Species Reactivity: Human
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