Human UCH-L3 / UCHL3 Protein, His Tag

DESCRIPTION

Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCH-L3), a member of peptidase C12 family, is also known as ubiquitin thioesterase L3. Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. UCH-L3 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is also required for stress-response retinal, skeletal muscle and germ cell maintenance. Furthermore, UCH-L3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.

DETAILS

Tag: N-6×His
Host: E. coli
Size: 100ug
Buffer: 50 mM Tris, 150 mM NaCl, pH7.5
Format: Powder
Purity: 95%
Source: Human UCH-L3, His Tag (UC3-H5141) is expressed from E. coli cells. It contains AA Glu 2 - Ala 230 (Accession # P15374-1).
Storage: -20℃
Category: Enzymes
Molecule: UCH-L3
Accession: AAH18125.1
Conjugate: Unconjugated
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Glu 2 - Ala 230
Swiss-prot: P15374-1
Package Size: 100ug*1
Characteristics: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 27.0 kDa
Shipping Condition: RT
Species Reactivity: Human
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