DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: poly(ADP-ribose)polymerase 2, poly(ADP-ribosyl)transferase 2, ADPRT, PARP2
• Warnings: Avoid freeze/thaw cycles.
• Category: PARP/Assay Kit
• References: Brown, J.A., Marala, R.B. 2002. J. Pharmacol. Toxicol. Methods 47:137-41.
• Description: The PARP2 Colorimetric Activity Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP2 biotinylated substrate is incubated with an assay buffer that contains the PARP2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.
• Methodology: Colorimetric
• Supplied As: The PARP2 assay kit comes in a convenient 96-well format, with purified PARP2 enzyme, histone mixture, activated DNA, and PARP2 assay buffer for 100 enzyme reactions.
• Unspsc Code: 41121811
• Unspsc Name: Laboratory microchemistry kits
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 80551, 80502, 80501, 80580, 78317
• Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: PARP
• Instructions for Use: See assay kit datasheet for detailed protocol.