Skip to Main Content
Welcome to the Scientist.com Marketplace

Go to Main Navigation

SH7-H5229-50ug SH7-H5229-1mg

Human Sonic Hedgehog / Shh Protein, His Tag, premium grade

ACROBiosystems

DETAILS

  • Tag: C-6×His
  • Host: HEK293
  • Size: 1mg
  • Buffer: PBS, pH7.4
  • Format: Powder
  • Purity: 90%
  • Storage: -20℃
  • Category: Cytokines&Growth Factors
  • Molecule: Sonic Hedgehog
  • Accession: NP_000184
  • Conjugate: Unconjugated
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: Sonic hedgehog protein (SHH) is also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
  • Exp Region: Cys 24 - Gly 197
  • Swiss-prot: Q15465
  • Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Endotoxin Level: 0.2 EU per μg
  • Molecular Weight: 20.4 kDa
  • Shipping Condition: RT
  • Species Reactivity: Human
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html

DESCRIPTION

Human Sonic Hedgehog, His Tag, premium grade (SH7-H5229) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184).