DESCRIPTION

• Category: REAGENTS
• Keywords: Z-Competent, competent cells, T3007, Z3007
• Paragraph 1: The Mix & Go E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium - Mix & Go! The premade Mix & Go competent cells are highly efficient (> 108 transformants / µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc. Premade Mix & Go competent cells strain Zymo 5a are supplied as a pack of 10 convenient single use aliquots, as a 96-tube rack with removable 8-tube strips, or as a 96-well PCR plate for your high-throughput transformation needs.
• Paragraph 2: Insert stability due to recA1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation. High plasmid yield due to endA1 mutation.
• Bullet Point 1: For general cloning, blue-white screening, plasmid isolation.
• Bullet Point 2: Mix & Go transformation procedure with transformation efficiencies of 10^8 - 10^9 transformants/µg of plasmid DNA.
• Bullet Point 3: Simple procedure: add DNA and then spread. DNA transformation in as little as 20 seconds!
• Manufacturer Sku: T3007