Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry. Cost-effective real-time qPCR master mix for probe-based qPCR assays. This master mix has been developed for TaqMan® probes but is suitable for other hydrolysis probe types as well. Suitable for singleplex and duplex assays High specificity and sensitivity Reaction set-up and shipment without dry ice HOT FIREPol® Probe qPCR Mix Plus (Capillary) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® Probe qPCR Mix Plus (Capillary) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity. HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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