GAS Luciferase Reporter Lentivirus (IFN-γ/JAK/STAT1 Pathway)
The GAS Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by three copies of the interferon gamma (IFN-γ) activated sites (GAS) located upstream of the minimal TATA promoter and a puromycin selection gene for the selection of stable clones. After transduction, the GAS-regulated gene expression in the target cells can be monitored by measuring the luciferase activity.
- Category: Lentivirus
- Subcategory: Cell Signaling Pathway
- Lead Time: 1-2 DAYS
- Applications: Screen for activators or inhibitors of IFN-γ-induced signal transduction pathways. Generate GAS Luciferase Reporter stable cell line (puromycin resistant).
- Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
- Background: Interferon gamma (IFN-γ) is a pleiotropic cytokine with anti-viral, anti-tumor, and immune-modulatory functions. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, Interferon gamma receptor 1 (IFNGR1) and Interferon gamma receptor 2 (IFNGR2), associated with kinases JAK1 and JAK2, respectively. Upon binding to this receptor, IFN-γ triggers JAK/STAT signaling. The activated STAT1 homodimers translocate to the nucleus where they bind interferon-gamma-activated sites (GAS) in the promoter of IFN-γ inducible genes, which stimulates IFN-γ-specific activation of gene transcription.
- Notes: To generate a GAS luciferase reporter stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of puromycin for antibiotic selection of transduced cells. Biosafety: The lentiviruses are produced with SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and after integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email email@example.com.
- Shiptemp: -80°C
- Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
- Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
- Unspsc Code: 41106621
- Unspsc Name: Virus mediated expression vectors or kits
- Warnings: Avoid freeze/thaw cycles.