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SPD-C82E3-25ug SPD-C82E3-200ug

Biotinylated SARS-CoV-2 Spike RBD Protein (L452R), His,Avitag™ (MALS verified)

ACROBiosystems

DESCRIPTION

Biotinylated SARS-CoV-2 Spike RBD Protein (L452R), His,Avitag (SPD-C82E3) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (L452R)). The L452R mutation was identified in the SARS-CoV-2 Epsilon variant (Pango lineage: B.1.427/B.1.429; other names: 20C/S:452R, or CAL.20C).

DETAILS

  • Tag: C-10×His & C-Avi
  • Host: HEK293
  • Size: 25ug
  • Buffer: PBS, pH7.4
  • Format: Powder
  • Purity: 90%
  • Storage: -20℃
  • Category: MABSol® Biotin Labeled Proteins
  • Molecule: Spike RBD
  • Accession: QHD43416.1 (L452R)
  • Conjugate: Biotin-labeled
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Exp Region: Arg 319 - Lys 537
  • Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.3 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Endotoxin Level: 1.0 EU per μg
  • Molecular Weight: 28.3 kDa
  • Shipping Condition: RT
  • Species Reactivity: SARS-CoV-2
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html