DESCRIPTION

• Target: BubR1 (pSer670)
• Gene Id: 701
• Conjugate: ATTO 594
• Immunogen: A phospho-specific peptide corresponding to residues surrounding Ser670 of human BUBR1 (AA667-673)
• Swiss-prot: O60566
• Specificity: Detects 119 kDa.
• Applications: WB | AM
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• Host Species: Rabbit
• Product Type: Polyclonal
• Purification: Peptide Affinity Purified
• Research Areas: Cancer | Cell Cycle | Cell Division | Cell Signaling | Epigenetics and Nuclear Signaling | Cell Signaling | Protein Phosphorylation
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.025% Thimerosal *Storage buffer may change when conjugated
• Accession Number: NP_001202
• Alternative Names: Beta homolg of S. cerevisiae BUB 1 antibody, Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody, BUB 1B antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody, Bub1A antibody, BUB1B antibody, BUB1B_HUMAN antibody, BUB1beta antibody, BUBR1 antibody, Budding Uninhibited by Benzimidazoles 1 beta antibody, Budding uninhibited by benzimidazoles 1 homolog beta (yeast) antibody, hBUBR1 antibody, MAD3/BUB1 related protein kinase antibody, MAD3L antibody, Mitotic checkpoint gene BUB1B antibody, Mitotic checkpoint kinase MAD3L antibody, MVA1 antibody, Protein SSK1 antibody, SSK 1 antibody, SSK1 antibody
• Antibody Dilution: WB (1:250); optimal dilutions for assays should be determined by the user.
• Cite This Product: StressMarq Biosciences Cat# SPC-929D-A594, RRID: AB_2707125
• Immunogen Species: Human
• Tissue Specificity: Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
• Storage Temperature: Conjugated antibodies should be stored according to the product label
• Shipping Temperature: Blue Ice or 4ºC
• Cellular Localization: Cytoplasm | Nucleus
• Scientific Background: BUBR1 is a protein-serine/threonine kinase. It is an essential component of mitotic checkpoint. It prevents progression of anaphase until all the sister chomatids are connected to spindles by binding to CDC20 to anaphase promoting complex/cycolsome thus inhibiting it. It is also involved in localization of CENPE, a kinetochore motor that performs kinetochore functions. Other functions include suppression of centrosome amplification, negative regulation of PLK1 in intephase and trigger apoptosis in polyploid cells.
• Certificate of Analysis: A 1:250 dilution of SPC-929 was sufficient for detection of BubR1 (pSer670) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Species Reactivity Full Name: Human