Biotinylated Human Complement C5 Protein, His,Avitag™

DESCRIPTION

Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

DETAILS

Tag: C-10×His & C-Avi
Host: HEK293
Size: 200ug
Buffer: PBS, pH7.4
Format: Powder
Purity: 95%
Source: Biotinylated Human Complement C5, His,Avitag (CO5-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1).
Storage: -20℃
Category: MABSol® Biotin Labeled Proteins
Molecule: Complement C5
Accession: NP_001726.2
Conjugate: Biotin-labeled
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Gln 19 - Cys 1676
Swiss-prot: P01031-1
Package Size: 200ug*1
Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 189.9 kDa. As a result of glycosylation, the protein migrates as 75 kDa, 120 kDa and 200 kDa under reducing (R) condition, and 200 kDa under non-reducing (NR) condition (SDS-PAGE).
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 189.9 kDa
Shipping Condition: RT
Species Reactivity: Human
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