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79980-G

Firefly Luciferase-eGFP Lentivirus (G418)

BPS Bioscience

DETAILS

  • Notes: License Disclosure: Visit bpsbioscience.com/license for the label license and other key information about this product.
  • Species: Replication incompetent HIV
  • Category: Lentivirus
  • Shiptemp: -80°C
  • Synonyms: pseudotyped lentivirus, pseudovirus, pseudovirion, 79980-G, 79980-P
  • Warnings: Biosafety: None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
  • Lead Time: 1-2 DAYS
  • Subcategory: Cell Signaling Pathway
  • Formulation: The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.
  • Supplied As: Two vials (500 µl x 2) of Firefly Luciferase-eGFP lentivirus at a titer ≥1 x 10^7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
  • Unspsc Code: 41106621
  • Unspsc Name: Virus mediated expression vectors or kits
  • Applications: Ideal as a positive control for transduction; useful for transduction optimization.
  • Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
  • Instructions for Use: See assay protocol for detailed instructions 

DESCRIPTION

The Firefly Luciferase-eGFP Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses contain a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) driven by a CMV promoter. Both the luciferase and eGFP are coexpressed under the CMV promoter in the transduced cells, allowing greater flexibility for detection of transduced cells.