32-1720
msRANKL Recombinant Protein
Abeomics
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DESCRIPTION
Source : Escherichia Coli.
sRANKL Mouse Recombinant produced in E.coli is single, non-glycosylated, polypeptide chain containing 174 amino acids & having a total molecular mass of 19.9kDa.CD254 is purified by proprietary chromatographic techniques.
RANKL binds to tnfrsf11b/opg & to tnfrsf11a/rank. Osteoclast differentiation & activation factor. augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells & dendritic cells & may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
DETAILS
- Amino Acid: PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID.
- Purification: Greater than 95.0% as determined by SDS-PAGE.
- Product Content: The protein (1mg/ml) was lyophilized with 10mM Na2PO4, pH 7.5 & 50mM NaCl.
- Alternative Name: Soluble Receptor Activator of NFkB Lig&||TNFSF11||TRANCE||TNF-related activation-induced cytokine||OPGL||ODF||Osteoclast differentiation factor||Tumor necrosis factor lig& superfamily member 11.
- Application Note: It is recommended to reconstitute the lyophilized sRANKL in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Measured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 50ng/ml shown in “Corning® Osteo Assay Surface 24 Well Plates with Transwell® Permeable Supports- A Useful Tool for Co-Culture Studies” by Rebecca M. Wood & Mark Rothenber, corresponding to a specific activity of 20,000Units/mg.
- Storage Condition: Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days & for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.