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79894-EH

TCR Activator Lentivirus (EF1a Promoter/Hygromycin)

BPS Bioscience

DETAILS

  • Category: Lentivirus
  • Shiptemp: -80°C
  • Warnings: None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local, federal, state, and institutional regulations and using all appropriate safety precautions.
  • Lead Time: 1-2 DAYS
  • Subcategory: Cell Signaling Pathway
  • Formulation: The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.
  • Supplied As: Two vials (500 µl x 2) of TCR Activator Lentivirus at a titer ≥10^7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
  • Unspsc Code: 41106621
  • Unspsc Name: Virus mediated expression vectors or kits
  • Applications: 1. Expression of TCR activator in target cells to stimulate T cells.2. Generation of a stable cell line expressing TCR activator with puromycin selection or hygromycin selection.
  • Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

DESCRIPTION

The TCR Activator Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a gene for a membrane-bound, engineered T cell receptor (TCR) activator driven by a CMV promoter or an EF1a promoter. After transduction, the expression of TCR activator can be monitored in the target cells.