DESCRIPTION

• Category: ZYMO KITS
• Keywords: D5031
• Paragraph 1: The EZ-96 DNA Methylation-Lightning™ Kit features high-throughput (96-well) bisulfite treatment and conversion of DNA for methylation analysis. Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is necessary, simply add this unique reagent to a DNA sample, wait about an hour, and let the reaction proceed to completion. DNA denaturation and bisulfite conversion processes are combined with added heat to facilitate rapid denaturation. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc.
• Bullet Point 1: Fastest method for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
• Bullet Point 2: Ready-to-use conversion reagent is added directly to DNA.
• Bullet Point 3: High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
• Bullet Point 4: Conversion Efficiency: >99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines.
• Bullet Point 5: Optimal DNA Input: Samples containing between 100 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng.
• Manufacturer Sku: D5031