Product Description MagVigen™ Plasma cfDNA Extraction Kit (Cat# K61003) is specially designed to capture, extract cell-free DNA (cfDNA), also called as circulating free DNA, from Plasma or Serum with no need of column or centrifugation. MagVigen™ cfDNA Extraction workflow can be scaled up linearly from 100 µl to 10 ml of plasma input samples. cfDNA isolated with MagVigen™ cfDNA Extraction kit presents high quality for downstream PCR or sequencing assays. The yield and quality of extracted cfDNA can be significantly better than other methods on market. Extracting cfDNA Workflow Lyse: Lyse plasma sample. Bind & Capture: Mix MagVigen™ nanoparticles with sample. Nanoparticles bind to the cfDNA fragments. Pellet & Re-disperse 1: Magnetic pellet the beads until the solution is clear. The beads respond to a magnetic force (by use of a magnet, e.g. magnetic separation rack), allowing bound material to be rapidly and efficiently separated from the rest of the sample. Remove supernatant (unbound materials) by aspiration, what remains is the nanoparticle-bound targets. Then re-disperse beads pellet in Wash 1 Buffer. Transfer: Transfer Wash 1 Solution to 1.5 or 2ml tube. Pellet & Re-disperse 2: Magnetic pelleting, remove supernatant as in step 3. This time re-disperse the beads pellet in 75% Ethanol. Wash & Pellet 2,3: Wash the beads pellet using 75% Ethanol. Pellet the beads and remove all supernatant. Re-disperse the beads pellet in 75% Ethanol. Do this step twice. Elute: Final elution of high quality cfDNA. Ready for downstream NGS, PCR or other Applications. 2 times of elution can help improve cfDNA yield.

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