DESCRIPTION

• Brand: Axis-shield
• Advantages: Advantages of OptiPrep™ vs Other gradients OptiPrep™ vs Histopaque & Percoll Percoll and Histopaque include one or several washing step(s) prior to their use. The removal step(s) is/are required to avoid the formation of density gradients which can be toxic to mammalian cells. Unlike Histopaque and Percoll, OptiPrep™ has no potential for bioaccumulation, therefore is non-toxic for the cell. OptiPrep™ has a better yield, purity and viability (92%, 86%, 54%) compared to Histopaque (58%, 61%, 54%). OptiPrep™ vs CsCl (Caesium chloride) Particle virus number ratios are 100 times higher with OptiPrep™ compared to those recorded from CsCl gradients.
• Brand Name: Axis Shield
• Applications: Cell Isolation, Isolation of subcellular organelles and membranes, Virus purification, Macromolecules and lipoproteins purification
• Publications: Publications mentioning OptiPrep™ 1. Wiesinger, C., Kunze, M., Regelsberger, G., Forss-Petter, S. and Berger, J. (2013) Impaired very long-chain Acyl-CoA ?-oxidation in human X-linked adrenoleukodystrophy fibroblasts is a direct consequence of ABCD1 transporter dyfunction J. Biol. Chem., 288, 19269-19279 2. Sandin, M., Antberg, L., Levander, F. and James, P. (2015) A Breast Cell Atlas: Organelle analysis of the MDAMB- 231 cell line by density-gradient fractionation using isotopic marking and label-free analysis EuPA Open Proteomics, 8, 68–77 3. Bagnat, M. and al. (2000) Lipid rafts function in biosynthetic delivery of proteins to the cell surface in yeast
• Related Products: • Lymphoprep: Isolation of human mononuclear cells • Lymphoprep™ Tube: Isolation of human mononuclear cells • Nycodenz AG : A universal density gradient medium • Polymorphprep: Isolation of human polymorphonuclear cells
• Shipping Details: Room Temperature
• Storage Condition: Storage and Stability Conditions of OptiPrep™ Should be stored between 4°C - 24°C in a sterile and dark environment. It can be stored up to 3 years. Avoid long exposures to sunlight ( NB. The effect of sunlight exposure is negligible when working with OptiPrep™ on a day to day basis). Before use invert the bottles several times to mix the contents.
• Delivery Condition: Room temperature
• Delivery Lead Time in Business Days: • Europe: 1-2 days • USA & Canada: 2-3 days • Rest of the World: 3-7 days