DESCRIPTION

• Category: GENE CHOICE KITS
• Keywords: 83-510, 83-511, 25-0500, 250500, 11-155, 15000-250, RNeasy Mini Kit, 74104, 74106, RNA purification, RNA kit, RNA kits, RNA clean up, RNA
• Paragraph 1: The Gene Choice® Total RNA Miniprep Kit from Genesee Scientific recovers up to 100µg of total RNA (>200nt) in 25 minutes from up to 30mg of mammalian tissue or cultured cells up to 1 x 10^7. The kit features a simple procedure with an RNA homogenizer column that combines sample homogenization and gDNA elimination into one step. Skip the need for toxic reagents like phenol and chloroform and lengthy precipitations. Purified RNA is eluted into 40-70µl and is ready for downstream applications such as NGS, RT-qPCR, RT-PCR, Northern Blotting, mRNA purification, nuclease protection and in vitro translation.
• Paragraph 2: DNase I set (cat # 96-107) is not included. Normally DNase I digestion is not required for RNA purified with the Gene Choice® Total RNA Isolation kit, as our silica-based spin column technology efficiently removes the majority of DNA without enzymatic digestion. However, certain sensitive RNA applications may require further DNA removal and the optional RNase-Free DNase I set is available for purchase separately.
• Bullet Point 1: Total RNA isolation from mammalian cells (1 x 10^7) or tissues up to 30mg
• Bullet Point 2: Purify total RNA >200nt in 25 minutes via centrifuge or vacuum protocols
• Bullet Point 3: Homogenizer column for integrated sample homogenization and gDNA removal in one step
• Bullet Point 4: Ultra-pure RNA ready for downstream applications such as RT-PCR, RT-qPCR, Northern Blotting, mRNA purification, etc.
• Bullet Point 5: Simple and safe procedure free of phenol and chloroform via centrifuge or vacuum manifold protocols
• Manufacturer Sku: 96-327