UCHL1 Antibody (C-term) Blocking peptide

DETAILS

Format: Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.
Gene Id: 7345
Category: Peptides; Blocking Peptides
Subtitle: Synthetic peptide
Gene Name: UCHL1
Other Names: Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1
Availability: 2 weeks
Bio Background: Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.
Bio References: Maraganore, D.M., et al., Mov Disord 18(6):631-636 (2003).Nishikawa, K., et al., Biochem. Biophys. Res. Commun. 304(1):176-183 (2003).Liu, Y., et al., Cell 111(2):209-218 (2002).Caballero, O.L., et al., Oncogene 21(19):3003-3010 (2002).Saigoh, K., et al., Nat. Genet. 23(1):47-51 (1999).
Primary Accession: P09936
Targetspecificity: The synthetic peptide sequence used to generate the antibody AP2126b was selected from the Park5 region of human UCHL1 (Park5) . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.