DESCRIPTION

• Notes: Biosaftey: The lentiviruses are produced with the third generation SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
• Shiptemp: -80°C (dry ice)
• Synonyms: SBE reporter, TGFβ reporter, TGFbeta reporter, SMAD reporter
• Warnings: Avoid freeze/thaw cycles.
• Category: Cell Line/Lentiviruses
• Background: The transforming growth factor beta (TGFβ) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGFβ signaling has been linked to cardiac disease, cancer, Alzheimer's and other human diseases. TGFβ proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGFβ/SMAD responsive genes.
• References: Moustakas A. et al. (2001) Smad regulation in TGF-beta signal transduction. J. Cell Science.114(Pt 24): 4359-69
• Description: The SBE Luciferase Reporter Lentivirus (TGFβ/SMAD signaling pathway) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized SBE-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the TGFβ/SMAD signaling pathway can be monitored by measuring the luciferase activity.
• Formulation: The lentiviruses were produced from HEK293T cells and are provided in medium containing 90% DMEM + 10% FBS.
• Supplied As: Two vials (500 µl x 2) of SBE luciferase reporter lentivirus at a titer ≥5 x 10^6 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
• Unspsc Code: 41106621
• Unspsc Name: Virus mediated expression vectors or kits
• Applications: • Screen for activators or inhibitors of the TGFβ/SMAD signaling pathway in transduced target cells• Generation of SBE Luciferase Reporter stable cell line
• Product Type: Lentivirus
• Biosafety Level: BSL-2
• Related Products: 90900-1, 60690, 79617, 79665
• Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
• Scientific Category: Cell Signaling Pathway
• Instructions for Use: See assay protocol for detailed instructions.